Brain death/death by neurologic criteria …

David M Greer MD

General Neurology

Updated 11.09.2024
Released 05.09.1994
Expires For CME 11.09.2027

Brain death/death by neurologic criteria

Author: David M Greer MD

See Contributor Disclosures

Editor: Matthew Lorincz MD PhD

Brain death/death by neurologic criteria

In This Article

Quick Link

Introduction

Overview

“Brain death,” or death by neurologic criteria (BD/DNC) is the common term for the determination of human death by showing the permanent cessation of all clinical brain functions. It has been accepted as a legal definition of death in the United States and throughout the world. BD/DNC must be determined by careful neurologic examination showing apnea, cranial nerve areflexia, and unresponsiveness that is permanent and caused by a structural lesion that accounts for the clinical findings. Tests showing the absence of intracranial blood flow can establish the diagnosis and should be used whenever any doubt arises over the clinical examination or if portions of it cannot be performed safely or adequately. In this article, the author discusses the need for uniformity in BD/DNC determination, updates to the 2023 American Academy of Neurology BD/DNC guidelines, the use of ancillary tests in BD/DNC, and several reports on specific topics, including BD/DNC in pregnancy, declaring BD/DNC in the setting of drug intoxication, BD/DNC in the setting of hypothermia or extracorporeal membrane oxygenation, the legal positions on BD/DNC, and portrayals of BD/DNC in the popular media.

Key points

	• BD/DNC is human death determined by establishing the permanent cessation of all clinical and physiologic functions of the brain.
	• BD/DNC is the legal standard for human death throughout the Western world and most of the developing world.
	• The essential criteria for BD/DNC are complete unresponsiveness (coma), brainstem areflexia, apnea, and permanence.
	• Tests showing the absence of intracranial circulation can confirm BD/DNC in cases in which the examination findings are drawn into question or the complete examination cannot be performed.

Historical note and terminology

"Brain death," or death by neurologic criteria, is the commonly accepted name for human death determined by clinical evaluation showing the permanent cessation of all clinical functions of the entire brain. The concept of BD/DNC originated with observations made by French neurologists in the late 1950s that patients with irreversible structural brain lesions maintained on ventilators showed an unprecedented depth of coma, cranial nerve areflexia, and apnea, a state they termed “coma dépassé,” meaning a “state beyond coma” (81). Their key findings included deep coma, lack of spontaneous breathing, inability to vary heart rate and blood pressure in response to appropriate stimuli, polyuria, and subsequent cardiac arrest days later. In a landmark paper in 1968, an ad hoc Harvard Medical School committee proposed the first definition of BD/DNC, which required the presence of coma (no response to external stimuli), no spontaneous movements or spontaneous breathing, lack of brainstem reflexes, and an isoelectric EEG (01). Since that time, guidelines for the determination of BD/DNC have been published, and the “Uniform Determination of Death Act” has been adopted by most states in the United States, establishing BD/DNC as a legal definition of death (102).

The term “brain death” is misleading because it incorrectly implies that only the brain has died and not the person. This term alone may be responsible for much of the confusion by the public and by some professionals. Yet because the term “brain death” has been generally accepted, it is likely to remain in common use. A more appropriate term is “death by neurologic criteria.” This article will use “BD/DNC” to accommodate both terms.

The prevalence of BD/DNC practice is expanding internationally. A 2002 survey of BD/DNC practices worldwide disclosed the accepted practice of BD/DNC determination in 80 countries surveyed. Practice guidelines were available in 70 of these countries; legal standards were in effect in 55 countries. Although there was widespread agreement on the concept of BD/DNC, differences were detected in the clinical practices of determining BD/DNC (133; 48). A detailed survey of international BD/DNC practices in 91 countries showed legal provisions in 70% and found that the presence of institutional BD/DNC protocols correlated highly with the presence of an organized organ transplantation network (126). Subsequent studies have demonstrated advances in the acceptance and practice surrounding BD/DNC in the United States and throughout the world (41; 66).

The terminology in this area is confusing. The old terms "cerebral death" and "neocortical death" should be abandoned because they incorrectly imply that the destruction of the cerebrum or neocortex alone is sufficient for death. The term "brainstem death" has been used in the United Kingdom to acknowledge that most of the bedside tests demonstrating the absence of clinical brain functions examine brainstem functions specifically (93). However, the term "brainstem death" adds confusion to an already confused terminology (80; 142) and should not be used in place of "brain death." Public surveys show persistent confusion about the meaning of the term brain death and its distinction from the persistent vegetative state (118; 72). Similarly, surveys of American neurologists disclosed an inadequate understanding of BD/DNC (54). Several countries continue to use the term “brainstem death” despite the confusion it introduces (119).

The concept of "whole BD/DNC" embodies the most useful and widely accepted criterion of death (04; 05; 07). Except for the United Kingdom, all BD/DNC clinical and ancillary tests in current use are based on the “whole brain” criterion of death, which requires that the clinical functions of all portions of the brain, including the brainstem, must have ceased irreversibly.

The American Academy of Neurology updated its evidence-based practice parameter for determining BD/DNC in 2023, combining adult and pediatric guidance into one document (02; 68). This was a joint effort with the Society of Critical Care Medicine, the American Association of Pediatrics, and the Child Neurology Society. This was a huge advance in the field, providing updated guidance for special circumstances, including BD/DNC in the setting of hypothermia, primary posterior fossa injury, pregnancy, and extracorporeal membrane oxygenation, among others. This came on the heels of the World Brain Death Project, published in JAMA in 2020 (41), a joint effort of 27 international societies to provide worldwide guidance and criteria.

There remains variability in how physicians determine BD/DNC. A study of the BD/DNC determination policies of the top 50 neurology departments in the United States revealed a disturbing pattern of non-uniformity (43). The authors found significant variations from accepted guidelines or from each other in many areas: who can perform the testing; whether more than one examination is required and the duration of the interval between examinations; the preconditions for testing (eg, establishing an underlying cause and requiring the exclusion of reversible metabolic or toxic factors, hypothermia, and shock); the techniques for testing brainstem function; whether and how to perform apnea testing; and whether and which ancillary electrophysiologic or neuroimaging tests are used. Similar results of practice variability were found in a later survey of 68 hospitals in the United States (113). A 2016 survey of 508 BD/DNC hospital policies in the United States showed significant noncompliance with the American Academy of Neurology standards, particularly by not excluding hypotension and hypothermia, and in the details of apnea testing and ancillary tests (44). The most recent survey showed progress in increasing the uniformity of BD/DNC determination guidelines among the leading United States medical centers in compliance with the American Academy of Neurology’s 2010 standards (128). The new AAN guidelines were published in 2023, and we can expect a follow-up study to look at variability in the coming years.

Not all scholars accept the conceptual validity of BD/DNC. A few physicians, philosophers, and theologians continue to hold that patients clinically determined to be BD/DNC are not truly dead (115; 116; 117). Some of these opponents to BD/DNC have argued that BD/DNC is not an entirely coherent concept, but these arguments have not persuaded medical or public policy bodies to stop recognizing BD/DNC as human death. BD/DNC continues to be an accepted legal standard of death throughout the majority of the Western and undeveloped world, and its practice is increasing in prevalence. In its analysis, the United States President’s Council on Bioethics acknowledged certain conceptual shortcomings in the concept of BD/DNC but recommended that BD/DNC continue to serve as a legal definition of death for the United States (President’s Council on Bioethics 2008).

The highly publicized BD/DNC cases in the United States sparked much public and scholarly commentary concerning whether they were truly dead and what the law stipulated (13; 08; 67). These discussions culminated in the identification of areas of uncertainty and the further work needed on BD/DNC (08).

Clinical manifestations

Presentation and course

BD/DNC should be suspected in any patient rendered deeply comatose and apneic from a profound diffuse brain insult. The cardinal diagnostic criteria for BD/DNC are: (1) the presence of a structural brain lesion sufficient to produce the clinical findings, (2) permanence by excluding potentially reversible metabolic and toxic factors, (3) profound coma with total unresponsiveness, (4) absence of all reflexes served by the brainstem and cranial nerves, and (5) apnea despite induced hypercapnia and acidosis (78; 131; 132; 16; 137; 85; 86; 41; 02). The authors recommend that BD/DNC determinations be performed in an intensive care unit setting where resources and subsequent discussions and care are most appropriate.

Presence of a structural brain lesion. Neuroimaging evidence of a catastrophic brain lesion (meaning one that will invariably cause loss of all cerebral blood flow and cessation of all brain function) is strongly recommended before initiating BD/DNC testing. If no adequate structural diffuse brain lesion can be identified, the clinician is obligated to consider alternate diagnoses and not declare BD/DNC (02).

Permanence. The demonstration of coma, apnea, and cranial nerve areflexia shows the absence of clinical brain functions. To show that their absence is permanent, the clinician must demonstrate that the lesion responsible for the patient's neurologic condition is structural and severe enough to cause cerebral circulatory arrest. Demonstration by CT or MR that the lesion is structural is easily accomplished in cases of massive head trauma or massive stroke. However, in cases of diffuse hypoxic-ischemic neuronal damage suffered during cardiopulmonary arrest, early CT or MR imaging may be normal or nondiagnostic, and delayed imaging may be required. A diffuse hypoxic-ischemic insult can be shown to be structural and permanent only if the patient satisfies BD/DNC criteria after an appropriate time interval and if all contributions from potentially reversible metabolic and toxic disorders can be excluded. Although BD/DNC declaration should be done expeditiously once a clinician suspects the diagnosis, there is potential for recovery of pupillary light reflexes in up to 30% of cardiac arrest patients, especially those treated with therapeutic hypothermia (22). As such, the guidelines recommend that in cardiac arrest patients who undergo therapeutic hypothermia, BD/DNC testing should be delayed for at least 24 hours post-rewarming, longer if there is concern for drug metabolism or other confounders. In overdose patients, consultation with a medical toxicologist or clinical pharmacist may be very helpful. If imaging findings already show diffuse cerebral edema with tonsillar herniation, the diagnosis of BD/DNC is probable, and delays in declaration may not be necessary.

A study questioned whether a second BD/DNC examination is necessary. In a review of 1229 adult and 82 pediatric BD/DNC determinations, Lustbader and colleagues showed that no patients with clinical findings of BD/DNC on the first examination regained any brain functions by the second examination (73). They also found, as expected, a negative impact on ICU bed utilization and a reduction in organ donation as a consequence of waiting for the second examination, which occurred after a mean interval of 19.9 hours. They concluded that in patients older than 1 year of age, a single examination was sufficient to determine BD/DNC. A second retrospective study conducted over 39 months by one of the investigators confirmed these findings in a separate patient population (125). A study of BD/DNC determinations in Canadian pediatric ICUs showed less than 1% discordance between the two examinations (1 in 110) (55).

The impact of these studies on clinical practice guidelines has been variable. The 2010 American Academy of Neurology BD/DNC guideline for adults no longer requires the second examination (137), but the 2011 multi-society guideline for diagnosing BD/DNC in infants and children continues to require two sequential examinations (85; 86). The 2023 AAN guidelines require one examination in adults but strongly encourage a second examination; only one apnea test is required in adults. In contrast, children (defined as 37 weeks gestational age to 18 years) require two examinations and two apnea tests, separated by 12 hours. Many state laws and hospital policies continue to mandate two serial examinations, sometimes requiring that they be performed by different physicians. Physicians declaring BD/DNC should know the requirements of their hospital policies and state laws.

A prerequisite for BD/DNC determination to exclude reversible causes is that significant levels of CNS-depressing drugs and neuromuscular blocking agents must be absent and that the patient is not severely hypothermic (temperature is less than 36°C). The patient must also be normotensive; per the new 2023 AAN guidelines, this means both a systolic blood pressure of at least 100 mm Hg and a mean arterial pressure of 75 mm Hg. These pharmaceutical agents and other confounders, as well as hypothermia, can induce a set of clinical findings similar to those seen in BD/DNC yet be potentially reversible with appropriate treatment. Gray and colleagues showed that usual therapeutic doses of neuromuscular blocking agents do not affect the integrity of pupillary light reflexes (40). No severe electrolyte, acid-base balance, or neuroendocrine disorder should be present that may be a reversible yet contributory factor to depressed CNS function. The American College of Medical Toxicology published a position statement on the determination of BD/DNC in the setting of drug overdose (88). They recommended that (1) drugs and toxins be identified by history and targeted testing; (2) at least five drug half-lives be considered an absolute minimum to assure drug clearance; and (3) a clinical or medical toxicologist be consulted in unclear situations. In patients with organ failure or those who have been subjected to hypothermia, five half-lives may not be sufficient for drug clearance, and the authors recommend either direct toxicology or ancillary testing.

The interfering role of therapeutic hypothermia in BD/DNC determination was dramatically reported in the case of a 55-year-old man who underwent therapeutic hypothermia after suffering cardiac arrest and was subsequently diagnosed as BD/DNC using accepted guidelines (130). However, when he arrived in the operating room for organ donation 24 hours later, he was observed to regain corneal reflexes, cough reflexes, and spontaneous respirations. This well-documented case of “reversible BD/DNC” shows that therapeutic hypothermia can induce a prolonged but potentially reversible metabolic encephalopathy that may violate the irreversibility condition for making a diagnosis of BD/DNC. One possible explanation is that the metabolism of depressant drugs administered before and during therapeutic hypothermia may be slowed by the hypothermia, so these drug effects are more prolonged than expected. Physicians caring for cardiac arrest patients who have undergone therapeutic hypothermia should delay BD/DNC determination for at least 24 hours after rewarming to 36°C until the reversible metabolic encephalopathy caused by hypothermia has abated. In the United States, primary brainstem injuries provide another diagnostic dilemma in the declaration of BD/DNC. Some patients who have suffered a direct injury to their brainstem will satisfy the examination criteria for BD/DNC, but they would not satisfy the “whole-brain” criteria as cortical perfusion and electrical activity may be spared initially. In these patients, it is now required in the 2023 AAN guidelines that there also be neuroimaging evidence of catastrophic supratentorial injury prior to proceeding with BD/DNC determination. Furthermore, in patients who have been hypothermic <35.5°C, either iatrogenically or due to environmental exposure, their temperature must be maintained at 36°C or greater for 24 hours before clinical testing can ensue.

Coma and unresponsiveness. Lack of a cerebrally-mediated response to external stimuli is required to declare BD/DNC. The patient should have no movements--no withdrawal or posturing (flexor or extensor), spontaneously or to noxious stimulation (including sternal rub, nailbed pressure, and noxious cranial stimuli, such as supra-orbital pressure). Because deep-tendon reflexes are integrated at a purely spinal cord level, their presence or absence is not directly relevant to brain functioning.

Spinally-mediated reflexes and automatisms are allowed in the diagnosis of BD/DNC. If reflexes are stimulus-induced, automatisms may occur spontaneously. These may include undulating toes, facial myokymia, multifocal spinal myoclonus, hugging-like movements, eyelid opening, or even head turning (51). A dramatic reflex known as the Lazarus sign occurs rarely in BD/DNC patients, usually during apnea testing. The patient may slowly elevate both arms and progressively adduct them across his or her chest. This bizarre sign is believed to result from involuntary motor discharges arising from previously intact cervical spinal cord anterior horn cells rendered progressively hypoxic during apnea testing (123). To prevent the misleading impression of intact brain function, family members should be informed of the presence of these movements and of their spinal origin to clarify that they are neither voluntary nor represent evidence of residual brain function.

Brainstem areflexia. Areflexia of those reflexes subserved by the cranial nerves must be present for BD/DNC to be determined. There can be no pupillary light or dark reflexes or oculovestibular, oculocephalic, corneal, gag, or cough reflexes. The absence of these reflexes must be ascertained accurately.

Pupillary light reflexes should be tested with a strong point-of-light source in a darkened room. A magnifying glass should be used in order to detect small pupillary changes, and a pupillometer may also be used as an adjunct measure, although this has not yet been validated for use in BD/DNC. There must be neither constriction to light nor dilatation to dark. Pupils are usually midposition (4 to 9 mm) because of sympathetic and parasympathetic denervation. Smaller pupil size (less than 2 mm) suggests a possible underlying drug effect, and potential confounding must be determined. Pupillary reflexes are unaffected by atropine given intravenously during resuscitation (36) and are also unaffected by therapeutic neuromuscular blockade during anesthesia (40). Midposition pupils of BD/DNC patients usually dilate further following circulatory arrest (64).

Corneal reflexes should be tested with a cotton-tipped applicator applied firmly to the cornea; reflexes must be absent. A sterile saline or water “squirt” may be used as a screening tool, but use of a cotton-tipped applicator with light pressure at the border of the iris is recommended to be definitive (75). Gag and cough reflexes can be assessed by suctioning the patient's endotracheal tube and stimulating the posterior pharynx bilaterally using a tongue depressor. Coughing or gagging resulting from pharyngeal or tracheal stimulation must be absent in BD/DNC.

Oculovestibular reflexes should be tested using the technique of "ice water caloric stimulation." An otoscopic examination is performed to ensure that the tympanic membranes are unruptured and that the external auditory canals permit passage of the ice water to the tympanic membranes. The patient's head is elevated to 30 degrees above horizontal. Through a flexible, dull-ended tube such as a butterfly plastic tube with the needle removed, 50 mL of ice water is infused over one minute into each external auditory canal as a second person holds open the patient's eyes. The BD/DNC patient should demonstrate no response to this test, making neither eye movements, limb movements, nor grimace (46). There should be an intervening time of at least 5 minutes before testing the opposite ear to allow the temperature of the endolymph to reequilibrate in the tested ear, and both sides must be tested.

Oculocephalic reflexes should be assessed only if spine and skull base integrity is ensured. Proper testing involves rapid movement of the head from side-to-side, and there should be no movement of the eyes in BD/DNC. If the oculocephalic reflex cannot be performed, this is the one part of BD/DNC clinical testing that can be omitted, provided that the oculovestibular test can still be fully performed.

Apnea. Apnea is a cardinal sign of BD/DNC, but many clinicians test for it incorrectly (26; 79). Lack of spontaneous respirations should be performed by disconnecting the ventilator. Merely turning off the ventilator for a few minutes and observing the absence of respiratory effort is an inadequate test for apnea because in all patients acidemia, not hypoxemia, is the primary stimulus to breathe. The PACO2 increases only approximately 3 mm Hg per minute of apnea; thus, observing a few minutes of apnea is an inadequate test (109). The technique, problems, and safety of apnea testing in BD/DNC have been reviewed (63; 111). It is worth noting that the 2023 AAN guidelines require both a PACO2 goal and a pH goal (< 7.30).

A proper apnea test must permit physicians to observe for respiratory effort in the setting of an elevated PACO2 while protecting the PAO2 from falling to dangerously low levels. Of the several strategies devised to achieve these goals, the most commonly used is the technique of apneic oxygenation. First, the ventilator settings are adjusted to bring the PACO2 to 35 to 45 mm Hg (assuming a baseline premorbid normocarbic patient) and a normal pH (7.35 to 7.45), and the patient is preoxygenated with an FiO2 = 1.0 (100% O2) to a PaO2 of greater than 200 mm Hg. At this point, passive oxygenation can be accomplished by placing the patient on blow-by or by inserting a cannula down the endotracheal tube to the level of the carina and infusing 100% O2 at 4 to 6 L per minute; higher flow rates may wash out CO2, making the determination uncertain. By starting with a markedly elevated PAO2 and passively oxygenating, despite 8 to 10 minutes of apnea, the PAO2 usually does not fall to dangerously low levels (03). The goal PACO2 is greater than 60 mm Hg and 20 above the baseline value, as well as pH less than 7.30. A study of BD/DNC determination from the Mayo Clinic confirmed that apnea testing is safe when performed using apneic oxygenation (136). In approximately 10% of BD/DNC determinations, apnea testing could not be performed, and ancillary testing was required to establish the diagnosis. The most common reason that apnea testing cannot be performed is when acute lung injury impairs the A-a gradient, blocking the rise of PAO2 to levels safe enough to conduct the examination (140).

The duration of apnea necessary to raise the PACO2 to greater than 60 mm Hg can be estimated by knowing the PACO2 when the apnea test was begun and using the rate of increase of PACO2 of approximately 3 mm Hg per minute of apnea. Generally, observing the patient during 7 minutes of apnea is sufficient to raise the PACO2 to greater than 60 mm Hg if the PACO2 was 40 mm Hg at the beginning of the test. Any respiratory effort, including sighing, counts as respiration and negates the apnea test. Major complications of the apnea test are hypotension, acidosis, cardiac arrhythmias, and hypoxemia. These complications are much more likely to occur in the setting of inadequate preoxygenation (53; 39). False negative apnea testing in BD/DNC may result from ventilator auto-triggering, also known as auto-cycling (23; 77). Respiratory therapy personnel assisting with apnea testing should be trained to recognize this phenomenon and not interpret it as indicative of brainstem-generated breathing.

In a patient with chronic hypercarbia in which their baseline PACO2 is known, that is the baseline value for that patient; they must achieve 20 mm Hg above that value during the test in addition to the pH < 7.30. In a patient with suspected chronic hypercarbia in which the baseline PACO2 is NOT known, it should be estimated, at the PACO2 values greater than 60 mm Hg and greater than20 mm Hg above the baseline, the pH <7.0, AND an ancillary test is required.

In the setting of ECMO, the 2023 AAN guidelines provide the following guidance:

	(1) Preoxygenate by using 100% FiO2 on the ventilator and through the membrane lung.
	(2) To achieve an adequate increase in PaCO2 level, either titrate exogenous CO2 into the ECMO circuit or adjust the sweep gas flow rate to 0.2 to 1 L/min.
	(3) Sample ABG measurements from both the patient’s distal arterial line and the ECMO circuit postoxygenator for patients on veno-arterial ECMO. Patients cannulated centrally, via the right carotid artery, or via the right axillary artery should have the distal arterial sample obtained from the left upper extremity or either lower extremity. Patients cannulated through the femoral artery should have the distal arterial sample obtained from the right upper extremity. PaCO2 and pH levels from both locations are required to meet BD/DNC criteria for the apnea test to be consistent with BD/DNC. This ensures that, independent of the mixing point, the PaCO2 and pH levels in the cerebral circulation meet BD/DNC criteria. For patients on venovenous ECMO, sample ABG measurements only from the patient’s distal arterial line.
	(4) Avoid hypotension during apnea testing on ECMO by increased ECMO flows, intravenous fluid administration, or vasopressor or ionotropic support.

Prognosis and complications

No patient declared BD/DNC by properly performed and interpreted tests has ever recovered. Generally, BD/DNC patients develop generalized vasodilatation because of destroyed medullary blood pressure centers, resulting in the development of severe hypotension requiring intravenous vasopressors and, often, high-output congestive heart failure (35). Usually, they also develop diabetes insipidus because of destruction of the hypothalamic neurons secreting antidiuretic hormone (92). Some BD/DNC patients do not develop diabetes insipidus, presumably because the independent circulation to the posterior hypothalamus may be preserved (84). Recently, the presence of renal dysfunction has been noted to impact the development of diabetes insipidus as well (124). Irrespective of treatment, most patients become asystolic within several days or weeks, but the heartbeat and circulation of a few young patients have remained functioning for months to even years. The major risks to transplantable organs in the BD/DNC donor that may require immediate treatment are pulmonary edema, hypotension, and polyuria (131). The effect of BD/DNC on systemic physiology has been reviewed (100).

Clinical vignette

A previously healthy 28-year-old man suffered a sudden, severe headache, vomited, and collapsed unconscious in a restaurant. He was taken to a hospital emergency room within 15 minutes, where he was intubated and ventilated. Examination revealed deep coma with no movements, no breathing above the set rate on the ventilator, absent brainstem reflexes, and weak bilateral decerebrate posturing to noxious stimuli. CT scan disclosed a massive intracranial hemorrhage originating in the left thalamus or basal ganglia with intraventricular rupture and probable bilateral uncal herniation.

He was treated with hyperventilation, intravenous mannitol, and vasopressin. There was no improvement on serial examinations at 4 hours and 12 hours, and the decerebrate posturing ceased. The neurologic consultant suggested incipient BD/DNC, and the organ procurement organization was notified for the possibility of organ donation. A formal BD/DNC evaluation was performed at 16 hours.

This examination disclosed unresponsiveness to all noxious stimuli. Ice water caloric irrigation of 50 cc in each external auditory canal was without oculovestibular reflex or other response. Pupillary, corneal, oculocephalic, gag, and cough reflexes were absent. Apnea testing disclosed no breathing effort after 10 minutes off the ventilator, with a PACO2 of 64 mm Hg and a pH of 7.27. Death was declared.

Autopsy restricted to the brain disclosed massive hemorrhage, bilateral uncal herniation, early autolysis, and remnants of an arteriovenous malformation.

Biological basis

Etiology and pathogenesis

In adults, BD/DNC is caused most commonly by massive head trauma, hypoxic-ischemic neuronal damage from prolonged cardiopulmonary arrest, and massive intracranial hemorrhage. In children, BD/DNC is produced most commonly by traumatic brain injury, bacterial meningitis, asphyxia, and drowning (121). In the largest reported study of 1844 BD/DNC patients in Spanish intensive care units, 42% had intracerebral hemorrhage, 19% had traumatic brain injury, and 14% had subarachnoid hemorrhage (27).

The pathogenesis of BD/DNC usually features three sequential phases. The first phase encompasses the primary brain insult, such as traumatic brain injury or diffuse neuronal injury from cardiopulmonary arrest. The edema produced by the primary insult raises intracranial pressure to a level greater than that of mean arterial blood pressure. At this point, intracranial blood flow ceases, and cerebral herniation occurs. During this second phase, the remainder of cerebral hemispheric, cerebellar, and particularly brainstem neurons not destroyed during the primary phase are killed as the result of complete cessation of intracranial blood flow. Thereafter, intracranial pressure falls, and during the third phase, there may be reperfusion of the infarcted brain (110).

In studies of the neuropathology of BD/DNC in the 1970s, the brain was autolyzed at autopsy. The severity of gross autolysis, however, is proportional to the duration of continued systemic normothermic circulation after BD/DNC has occurred (127). Therefore, relatively little gross evidence of autolysis may be present if the BD/DNC determination has been rapid. A study of the pathology of BD/DNC confirmed that, in the contemporary era, the autolysis seen in studies from the 1970s usually no longer is present because the patients are declared dead within a day or two of the global brain injury and circulation ceases before the gross pathological changes have had time to occur (135).

The widespread physiological and neuroendocrine abnormalities produced by BD/DNC have been studied in patients undergoing multiorgan procurement (98; 19).

Differential diagnosis

BD/DNC should be suspected when a patient has been rendered comatose and apneic and the brainstem rendered areflexic by a permanent, diffuse, structural brain insult. If the patient satisfies the BD/DNC criteria previously listed, it is unlikely that any other diagnosis is responsible for the patient’s examination findings; the patient is BD/DNC and is declared dead. Other differential diagnoses of coma and brainstem deficits should be considered if patients do not satisfy all of the previously described criteria. (97).

There are several pitfalls in the diagnosis that the experienced examiner should carefully avoid (14). The examination findings of BD/DNC can be produced by drug intoxication or profound neuromuscular blockade, such as from delayed vecuronium clearance. Therefore, the absence of these drugs in concentrations sufficient to produce physical signs is a prerequisite for BD/DNC determination (58; 91). Occasional cases of severe Guillain-Barré syndrome or botulism causing profound paralysis may mimic many of the findings in BD/DNC (49; 34; 33).

Pupillary light reflexes, oculovestibular reflexes, and gag reflexes may be absent because of preexisting disease. Pupillary light reflexes rarely can be affected by ingested or prescribed medications, such as atropine. Oculovestibular reflexes can be abolished by aminoglycoside antibiotics. Patients with CO2-retaining chronic obstructive pulmonary disease may have false-positive apnea tests because they may breathe based more on their hypoxemic drive and not on their hypercapnic drive. Ventilator auto-cycling despite true apnea can falsely suggest an intact respiratory drive. This phenomenon, if present, should be evaluated by experienced respiratory therapists and anesthesiologists (134; 23; 77).

The vegetative state should be distinguished from BD/DNC. Patients in the vegetative state have eyes-open unawareness resulting from profound dysfunction of the cerebral hemispheres, yet these patients have retained sleep-wake cycles and variably intact brainstem functions, including breathing and cranial nerve reflexes (82; 52; 06).

Diagnostic workup

Algorithms to guide the less-experienced clinician in the determination of BD/DNC are available (41; 02; 68). Only experienced clinicians should perform BD/DNC determinations; trainees and advanced practice practitioners may perform BD/DNC testing only under the direct supervision of an attending physician unless otherwise stipulated by hospital policy. Non-neurologists, including intensivists and neurosurgeons, should also undergo proper training, which may include simulation courses (74) or online courses, such as those provided through the Neurocritical Care Society.

Several centers have produced simulation training programs to teach accurate BD/DNC determinations (74; 47). One advantage of simulation training is learning to overcome confounders and pitfalls in BD/DNC determination and to learn the best ways to explain BD/DNC to family members.

It is essential for the clinician not only to fully and carefully examine the patient but also to carefully record the clinical findings that support the diagnosis of BD/DNC. One study of 121 BD/DNC chart notes disclosed the disturbing findings that physicians recorded the results of tests of pupillary reflexes in only 86%, gag reflexes in 78%, and corneal reflexes in 57% of cases (129). Another study showed a surprisingly high variability rate of hospital policies for BD/DNC determination (101). A study of pediatric BD/DNC determination among hospitals in southern California showed a disturbing pattern of variability and lack of compliance with accepted test batteries (76). The authors advocated requiring physicians to complete a checklist when determining BD/DNC. Completing a checklist ensures that no examination element is omitted and that the clinician has made adequate documentation of the absent signs to justify the declaration of death. Both the 2020 World Brain Death Project and the 2023 AAN guidelines provided useful checklists (41; 02; 68). The role of laboratory and radiological evaluations is to confirm the bedside diagnosis of BD/DNC and to investigate the state of brain structure and function. Ancillary tests may be useful to supplement the clinical examination in three situations: (1) when an adequate clinical examination or apnea test cannot be performed, such as in states of trauma when the eyes, tympanic membranes, or lungs have been damaged; (2) in medicolegal circumstances in which the clinician wishes to have "objective" evidence to submit in addition to his or her own bedside examination findings; and (3) when concurrent metabolic or toxic factors may be affecting the clinical findings and cannot be corrected. In the last case, it is important to note that the entirety of the clinical examination, including the apnea test, still needs to be performed, and if signs of brain function are present, the patient is clearly not BD/DNC, and ancillary testing is not indicated. The ancillary test chosen will depend primarily on which test is available and the stability of the patient. Both the World Brain Death Project and the 2023 AAN guidelines downgraded electroencephalography (EEG), as it does not measure brainstem function or integrity. The authors recommend that a detailed clinical evaluation of BD/DNC criteria be performed and documented before starting ancillary tests, as these should only be undertaken if there is sufficient clinical evidence. Ancillary tests of BD/DNC now include only tests of cerebral blood flow. A survey of neurointensivists showed that radionuclide blood flow studies are used most frequently, and younger physicians are more likely to use transcranial Doppler ultrasound (11). Note that there remains variability among countries as to which ancillary tests are acceptable, and the reader is encouraged to investigate these accordingly.

Cerebral blood flow ancillary tests showing the absence of intracranial blood flow include catheter-based cerebral angiography (37; 32; 24; 143), single-photon emission computed tomography (SPECT) perfusion imaging with 99m technetium-hexamethylpropylene amineoxime (HMPAO) (105; 138; 141; 89; 83), and transcranial Doppler ultrasonography (99). Neither CT angiography nor MRI angiography has been validated for use as an ancillary test in BD/DNC, and both the World Brain Death Project and the 2023 AAN guidelines recommend against their use. Each of these tests exploits the fact that all intracranial blood flow ceases at some time during the process of BD/DNC.

Transcranial Doppler ultrasonography is the simplest of the intracranial blood flow tests and demonstrates reliable findings in BD/DNC. It is especially useful for patients deemed too unstable to transport out of the intensive care unit. A transcranial Doppler ultrasound test confirms BD/DNC if one of the following patterns is present: sharp systolic peaks when intracranial pressure exceeds systolic blood pressure and "reverberating" blood flow with reversal of flow or zero values during diastole when intracranial pressure exceeds diastolic blood pressure but is lower than systolic blood pressure (94; 25). Proper testing requires insonation both anteriorly and posteriorly and bilaterally, with two examinations separated by at least 30 minutes. Cranial insonation may be performed transorbitally with a high success rate (62). A meta-analysis of 22 studies found that transcranial Doppler ultrasound is a highly accurate ancillary test for BD/DNC with a pooled sensitivity of 90% and a pooled specificity of 98% (18).

A few studies exist comparing the relative reliability of the tests confirming BD/DNC. In a 2008 review, Heran and colleagues advocated for HMPAO-SPECT as the ancillary test with the highest positive and negative predictive values for BD/DNC (45). Two studies showed detectable blood flow imaged by CTA when the patient was clinically BD/DNC, and the EEG was flat (104; 60). Another case report showed absent blood flow by CTA when intracranial flow was detected by transcranial Doppler (42).

Some data on physicians’ practice preferences regarding ancillary testing are available. Radionuclide angiography is ordered most commonly in practice, but younger physicians prefer transcranial Doppler ultrasound (11). There is evidence that neurosurgeons and critical care physicians performing BD/DNC determinations rely more on intracranial blood flow tests, and neurologists rely more on clinical tests (87).

Management

BD/DNC patients should be declared dead and not treated with the goal of trying to alter the patient’s outcome. Exceptions include temporary treatment for prospective organ donors prior to procurement (21), patients whose religions preclude BD/DNC determinations (15; 107), and some pregnant women in whom the decision has been made to maintain oxygenation and circulation temporarily to permit the subsequent birth of the infant (28). Shaner and colleagues have published practical guidelines from the California Medical Association Ethics Committee for optimally implementing BD/DNC examinations and requests for organ procurement in clinical practice (112). The Canadian Medical Association published similar guidelines (114). However, several complications associated with herniation may require medical management either before the declaration of BD/DNC or in preparation for organ procurement in the BD/DNC donor.

Hemodynamic instability. Blood pressure and heart rate variability are commonly encountered in BD/DNC due to autonomic dysregulation from injury to sympathetic pathways. Patients frequently require vasopressors to maintain a systolic blood pressure above 100 mmHg and a mean arterial pressure above 75 mm Hg, which is a prerequisite for the determination of BD/DNC. Vasopressors frequently used include norepinephrine, dopamine, phenylephrine, and epinephrine (59). Tachyarrhythmias may also be seen, and treatment should be aimed at correction of the precipitating factor (139). The authors recommend short-acting therapies to avoid reflex bradycardia. Bradyarrhythmias occurring in the setting of herniation and BD/DNC tend to be refractory to atropine and may require epinephrine (139).

Endocrine dysfunction. Pituitary dysfunction may lead to endocrine derangements such as diabetes insipidus. In BD/DNC patients who develop significant polyuria, the subsequent hypovolemia may worsen hypotension. In patients who meet the criteria for diabetes insipidus (elevated urine output, hypernatremia with sodium greater than 145mEq, decreased urine osmolality with specific gravity less than 1.005), appropriate fluid repletion and hormonal replacement are required. We recommend desmopressin (ddAVP) intravenously or vasopressin continuous infusion if urine output exceeds 300 mL/hr. The patient’s urine output should also be replaced with equal volumes of a hypotonic solution, such as half normal saline. Serum sodium and urine-specific gravity should be checked frequently to ensure titration of fluids and vasopressin are adequate.

Legal considerations. Laws and regulations in the United States require physicians to offer organ and tissue donation to families of patients declared dead. The benefits to society of increasing the organ pool are obvious, especially given the growing disproportion between the number of patients who need organs and the inadequate donor organ pool. It is clear that families of organ donors benefit as a result of the meaning conferred by the gift of the organ from the dead patient that maintains the life or health of the organ recipient; however, how the organ donation consent discussion with families should be optimally conducted remains controversial, particularly the role of the organ procurement organization staff (106). It is imperative that the family not be given the impression that the opportunity for organ donation is driving the BD/DNC determination and that valid informed consent is obtained for organ donation (50).

All states in the United States have either enacted statutes of death or drafted administrative regulations supporting the use of BD/DNC as a legal determination of death (96). Similar laws exist in most other Western and industrialized nations (52). In cases where the validity of BD/DNC determination has become the subject of judicial review, all courts in the United States have upheld the practice of BD/DNC (12). California, New Jersey, and New York have “accommodation clauses” that families can incur in order to continue life support in BD/DNC patients. Physicians who practice in states where such religious exemptions have been enacted should familiarize themselves with these laws (95).

Physicians should be sensitive to the emotional needs of family members, particularly in those cases in which family members request or demand continued treatment. These demands have been reported by nearly half of American neurologists in a survey (65). One reason commonly noted is the belief of family members that the BD/DNC loved one will recover if treatment is continued. This false belief may be furthered by misleading or incorrect depictions of BD/DNC patients in film and television and in the print media (20; 71). Physicians faced with such cases should consult the thoughtful summary of practical guidelines for their management by Flamm and colleagues (31). Techniques to enhance communication between physicians and family members can increase family member understanding of BD/DNC. When implemented, they improve the quality of end-of-life care. One technique that enhances family acceptance is to allow the family members to observe the BD/DNC determination (90). However, one trial of this technique in practice failed to improve family acceptance (57). A review of American hospital BD/DNC protocols showed that 80% provided no guidance on how to resolve disputes with family members over BD/DNC (70).

Special considerations

Pregnancy

Several cases have been reported of BD/DNC pregnant women whose heartbeat and circulation were maintained temporarily to permit the birth of a healthy fetus. These cases raise interesting ethical and legal problems of whether the continued therapy is justified, as well as challenging technical problems of maintaining physiological functions in the absence of clinical brain functions (30; 10; 56; 17; 120; 29; 28). One survey showed that BD/DNC in pregnancy was not provided for in hospital BD/DNC policies (69). The investigators found that 2.5% of hospital policies eliminated pregnant patients from BD/DNC consideration and that, in the remainder, which did permit BD/DNC determination, only 6.2% provided guidance in this situation and only 1% indicated who was responsible for making decisions for the fetus. The AAN provided a position statement regarding both pregnancy and accommodation in 2019 (108), and the 2023 AAN guidelines state, “Pregnancy in and of itself is not a contraindication to BD/DNC evaluation. Clinicians should assess and diagnose pregnant persons with catastrophic, permanent brain injuries for BD/DNC.” They further state that “After the determination of BD/DNC in a pregnant person, the clinicians providing care, assisted by clinicians knowledgeable in maternal-fetal medicine, child neurology, and neonatology, as needed, should educate and discuss with surrogate decision-makers the risks and benefits to the fetus of continuing maternal organ support.”

Sperm retrieval

One report described a BD/DNC man from whom viable sperm were retrieved by the technique of electro-ejaculation (122).

Anesthesia

General anesthesia is not necessary when BD/DNC patients undergo organ donation because there is no possibility of pain perception or suffering.

References

01: Ad Hoc Committee. A definition of irreversible coma. Report of the Ad Hoc Committee of the Harvard Medical School to Examine the Definition of Brain Death. JAMA 1968;205:337-40. PMID 5694976
02: Anonymous. Pediatric and Adult Brain Death/Death by Neurologic Criteria Consensus Guideline: Report of the AAN Guidelines Subcommittee, AAP, CNS, and SCCM. Neurology 2024;102(3):e208108. PMID 38175986
03: Belsh JM, Blatt R, Schiffman PR. Apnea testing in brain death. Arch Intern Med 1986;146:2385-8. PMID 3778074
04: Bernat JL. A defense of the whole-brain concept of death. Hastings Cent Rep 1998;28(2):14-23. PMID 9589289
05: Bernat JL. The concept and practice of brain death. Prog Brain Res 2005;150:369-79. PMID 16186036
06: Bernat JL. Chronic disorders of consciousness. Lancet 2006;367:1181-92. PMID 16616561
07: Bernat JL. Contemporary controversies in the definition of death. Prog Brain Res 2009;177:21-31. PMID 19818892
08: Bernat JL. Whither brain death? Am J Bioeth 2014;14(8):3-8. PMID 25046285
09: Bernat JL, Larriviere D. Areas of persisting controversy in brain death. Neurology 2014;83(16):1394-5. PMID 25217054
10: Bernstein IM, Watson M, Simmons GM, Catalano PM, Davis G, Collins R. Maternal brain death and prolonged fetal survival. Obstet Gynecol 1989;74:434-7. PMID 11655965
11: Boissy AR, Provencio JJ, Smith CA, Diringer MN. Neurointensivists’ opinions about death by neurological criteria and organ donation. Neurocrit Care 2005;3:115-21. PMID 16174879
12: Burkle CM, Schipper AM, Wijdicks EF. Brain death and the courts. Neurology 2011;76(9):837-41. PMID 21357836
13: Burkle CM, Sharp RR, Wijdicks EF. Why brain death is considered death and why there should be no confusion. Neurology 2014;83(16):1464-9. PMID 25217058
14: Busl KM, Greer DM. Pitfalls in the diagnosis of brain death. Neurocrit Care 2009;11(2):276-87. PMID 19444652
15: Byrne PA, O'Reilly S, Quay PM. Brain death—an opposing viewpoint. JAMA 1979;242:1985-90. PMID 480645
16: Canadian Neurocritical Care Group. Guidelines for the diagnosis of brain death. Can J Neurol Sci 1999;26:64-6. PMID 10068812
17: Catanzarite VA, Willms DC, Holdy KE, Gardner SE, Ludwig DM, Cousins LM. Brain death during pregnancy: tocolytic therapy and aggressive maternal support on behalf of the fetus. Am J Perinatol 1997;14:431-4. PMID 9263566
18: Chang JJ, Tsivgoulis G, Katsanos AH, Malkoff MD, Alexandrov AV. Diagnostic accuracy of transcranial Doppler for brain death confirmation: systemic review and meta-analysis. AJNR Am J Neuroradiol 2016;37(3):408-14. PMID 26514611
19: Cooper DK, Basker M. Physiologic changes following brain death. Transplant Proc 1999;31:1001-2. PMID 10083445
20: Daoust A, Racine E. Depictions of brain death in the media: medical and ethical implications. J Med Ethics 2014;40(4):253-9. PMID 23584826
21: Darby JM, Stein K, Grenvik A, Stuart SA. Approach to management of the heartbeating 'brain-dead' organ donor. JAMA 1989;261:2222-8. PMID 2648042
22: Dhakal LP, Sen A, Stanko CM, et al. Early absent pupillary light reflexes after cardiac arrest in patients treated with therapeutic hypothermia. Ther Hypothermia Temp Manag 2016;6(3):116-21. PMID 27135180
23: Dodd-Sullivan R, Quirin J, Newhart J. Ventilator autotriggering: a caution in brain death diagnosis. Prog Transplant 2011;21(2):152-5. PMID 21736245
24: Donohoe KJ, Agrawal G, Frey KA, et al. SNM practice guideline for brain death scintigraphy 2.0. J Nucl Med Technol 2012;40(3):198-203. PMID 22743146
25: Ducrocq X, Braun M, Debouverie M, Junges C, Hummer M, Vespignani H. Brain death and transcranial Doppler: experience in 130 cases of brain-dead patients. J Neurol Sci 1998;160:41-6. PMID 9804115
26: Earnest MP, Beresford HR, McIntyre HB. Testing for apnea in brain death: methods used by 129 clinicians. Neurology 1986;36:542-4. PMID 3960329
27: Escudero D, Valentin MO, Escalante JL, et al. Intensive care practices in brain death diagnosis and organ donation. Anaesthesia 2015;70(10):1130-9. PMID 26040194
28: Esmaeilzadeh M, Dictus C, Kayvanpour E, et al. One life ends, another begins: management of a brain-dead pregnant mother-a systematic review-. BMC Med 2010;8:74. PMID 21087498
29: Farragher RA, Laffey JG. Maternal brain death and somatic support. Neurocrit Care 2005;3:99-106. PMID 16174876
30: Field DR, Gates EA, Creasy RK, Jonsen AR, Laros RK Jr. Maternal brain death during pregnancy. Medical and ethical issues. JAMA 1988;260:816-22. PMID 3392814
31: Flamm AL, Smith ML, Mayer PA. Family members’ requests to extend physiologic support after declaration of brain death: a case series analysis and proposed guidelines for management. J Clin Ethics 2014;25(3):222-37. PMID 25192347
32: Flowers WM Jr, Patel BR. Radionuclide angiography as a confirmatory test for brain death: a review of 229 studies in 219 patients. South Med J 1997;90:1091-6. PMID 9386049
33: Freund B, Hayes L, Rivera-Lara L, et al. Adult intestinal colonization botulism mimicking brain death. Muscle Nerve 2017;56(4):E27-8. PMID 28500638
34: Friedman Y, Lee L, Wherrett JR, Ashby P, Carpenter S. Simulation of brain death from fulminant de-efferentation. Can J Neurol Sci 2003;30:305-6. PMID 14672276
35: Fugate JE, Rabinstein AA, Wijdicks EF. Blood pressure patterns after brain death. Neurology 2011;77(4):399-401. PMID 21753170
36: Goetting MG, Conteras E. Systemic atropine administration during cardiac arrest does not cause fixed, dilated pupils. Ann Emerg Med 1991;20:55-7. PMID 1984729
37: Goodman JM, Heck LL, Moore B. Confirmation of brain death with portable isotope angiography: a review of 204 consecutive cases. Neurosurgery 1985;16:492-7. PMID 3990928
38: Gore SM, Cable DJ, Holland AJ. Organ donation from intensive care units in England and Wales: two year confidential audit of deaths in intensive care. BMJ 1992;304:349-55. PMID 1540732
39: Goudreau JL, Wijdicks EF, Emery SF. Complications during apnea testing in the determination of brain death: predisposing factors. Neurology 2000;55:1045-8. PMID 11061269
40: Gray AT, Krejci ST, Larson MD. Neuromuscular blocking drugs do not alter the pupillary light reflex of anesthetized humans. Arch Neurol 1997;54:579-84. PMID 9152114
41: Greer DM, Shemie SD, Lewis A, et al. Determination of brain death/death by neurologic criteria: the world brain death project. JAMA 2020;324(11):1078-97. PMID 32761206
42: Greer DM, Strozyk D, Schwamm LH. False positive CT angiography in brain death. Neurocrit Care 2009;11(2):272-5. PMID 19390993
43: Greer DM, Varelas PN, Haque S, Wijdicks EF. Variability of brain death determination guidelines in leading US neurologic institutions. Neurology 2008;70(4):284-9. PMID 18077794
44: Greer DM, Wang HH, Robinson JD, Varelas PN, Henderson GV, Wijdicks EF. Variability of brain death policies in the United States. JAMA Neurol 2016;73(2):213-8. PMID 26719912
45: Heran MK, Heran NS, Shemie SD. A review of ancillary tests in evaluating brain death. Can J Neurol Sci 2008;35:409-19. PMID 18973057
46: Hicks RG, Torda TA. The vestibulo-ocular (caloric) reflex in the diagnosis of cerebral death. Anaesth Intensive Care 1979;7:169-73. PMID 507352
47: Hocker S, Schumacher D, Mandrekar J, Wijdicks EF. Testing confounders in brain death determination: a new simulation model. Neurocrit Care 2015;23(3):401-8. PMID 25898887
48: Hornby K, Shemie SD, Teitelbaum J, Doig C. Variability in hospital-based brain death guidelines in Canada. Can J Anaesth 2006;53:613-9. PMID 16738298
49: Hughes R, McGuire G. Neurologic disease and the determination of brain death: the importance of a diagnosis. Crit Care Med 1997;25:1923-4. PMID 9366780
50: Iltis AS. Organ donation, brain death and the family: valid informed consent. J Law Med Ethics 2015;43(2):369-82. PMID 26242960
51: Jain S, DeGeorgia M. Brain death-associated reflexes and automatisms. Neurocrit Care 2005;3:122-6. PMID 16174880
52: Jennett B. The vegetative state: medical facts and legal dilemmas. Cambridge: Cambridge University Press, 2002.
53: Jeret JS, Benjamin JL. Risk of hypotension during apnea testing. Arch Neurol 1994;51:595-9. PMID 8198471
54: Joffe AR, Anton NR, Duff JP, Decaen A. A survey of American neurologists about brain death: understanding the conceptual basis and diagnostic tests for brain death. Ann Intensive Care 2012;2(1):4. PMID 22339807
55: Joffe AR, Shemie SD, Farrell C, Hutchison J, McCarthy-Tamblyn L. Brain death in Canadian PICUs: demographics, timing, and irreversibility. Pediatr Crit Care Med 2013;14(1):1-9. PMID 23132398
56: Kantor JE, Hoskins IA. Brain death in pregnant women. J Clin Ethics 1993;4:308-14. PMID 7803825
57: Kompanje EJ, de Groot YJ, Bakker J, Ijzermans JN. A national multicenter trial on family presence during brain death determination: the FABRA study. Neurocrit Care 2012;17(2):301-8. PMID 21989843
58: Korein J. The diagnosis of brain death. Semin Neurol 1984;4:52-72. PMID 11649668
59: Kotloff RM, Blosser S, Fulda GJ, et al. Management of the potential organ donor in the ICU: Society of Critical Care Medicine/ American College of Chest Physicians/Association of Organ Procurement Organizations consensus statement. Crit Care Med 2015;43:1291-325. PMID 25978154
60: Kramer AH, Roberts DJ. Computed tomography angiography in the diagnosis of brain death: a systematic review and meta-analysis. Neurocrit Care 2014;21(3):539-50. PMID 24939056
61: Kramer AH, Zygun DA, Doig CJ, Zuege DJ. Incidence of neurologic death among patients with brain injury: a cohort study in a Canadian health region. CMAJ 2013;185(18):E838-45. PMID 24167208
62: Lampl Y, Gilad R, Eschel Y, et al. Diagnosing brain death using the transcranial Doppler with a transorbital approach. Arch Neurol 2002;59:58-60. PMID 11790231
63: Lang CJ, Heckmann JG. Apnea testing for the diagnosis of brain death. Acta Neurol Scand 2005;112:358-69. PMID 16281917
64: Larson MD, May J. Effect of asphyxia on the pupils of brain-dead subjects. Resuscitation 2002;55:31-6. PMID 12297351
65: Lewis A, Adams N, Varelas P, Greer D, Caplan A. Organ support after death by neurological criteria: results of a survey of US neurologists. Neurology 2016c;87(8):827-34. PMID 27449064
66: Lewis A, Bakkar A, Kreiger-Benson E, et al. Determination of death by neurologic criteria around the world. Neurology 2020;95(3):e299-309. PMID 32576632
67: Lewis A, Cahn-Fuller K, Caplan A. Shouldn’t dead be dead? The search for a uniform definition of death. J Law Med Ethics 2017b;45:112-28. PMID 28661278
68: Lewis A, Kirschen MP, Greer D. The 2023 AAN/AAP/CNS/SCCM Pediatric and Adult Brain Death/Death by Neurologic Criteria Consensus Practice Guideline: a comparison with the 2010 and 2011 guidelines. Neurol Clin Pract 2023;13(6):e200189. PMID 37829552
69: Lewis A, Varelas P, Greer D. Pregnancy and brain death: lack of guidance in U.S. hospital policies. Am J Perinatol 2016a;33(14):1382-7. PMID 27159202
70: Lewis A, Varelas P, Greer D. Prolonging support after brain death: when families ask for more. Neurocrit Care 2016b;24(3):481-7. PMID 26490777
71: Lewis A, Weaver J, Caplan A. Portrayal of brain death in film and television. Am J Transplant 2017a;17(3):761-9. PMID 27642118
72: Long T, Sque M, Addington-Hall J. What does a diagnosis of brain death mean to family members approached about organ donation. A review of the literature. Prog Transplant 2008;18:118-25. PMID 18615977
73: Lustbader D, O’Hara D, Wijdicks EF, et al. Second brain death examination may negatively affect organ donation. Neurology 2011;76(2):119-24. PMID 21172836
74: MacDougall BJ, Robinson JD, Kappus L, Sudikoff SN, Greer DM. Simulation-based training in brain death determination. Neurocrit Care 2014;21(3):383-91. PMID 24692109
75: Maciel CB, Youn TS, Barden MM, et al. Corneal reflex testing in the evaluation of a comatose patient: an ode to precise semiology and examination skills. Neurocrit Care 2020;33(2):399-404. PMID 31919808
76: Mathur M, Petersen L, Stadtler M, et al. Variability in pediatric brain death determination and documentation in southern California. Pediatrics 2008;121:988-93. PMID 18450904
77: McGee WT, Mailloux P. Ventilator autocycling and delayed recognition of brain death. Neurocrit Care 2011;14(2):267-71. PMID 21222050
78: Medical Consultants to the President's Commission. Guidelines for the determination of death. Report of the medical consultants on the diagnosis of death to the President's Commission for the Study of Ethical Problems in Medicine and Biomedical and Behavioral Research. JAMA 1981;246:2184-6. PMID 7289009
79: Mejia RE, Pollack MM. Variability in brain death determination practices in children. JAMA 1995;274:550-3. PMID 7629983
80: Molinari GF. Brain death, irreversible coma, and words doctors use. Neurology 1982;32:400-2. PMID 7199662
81: Mollaret P, Goulon M. Le coma dépassé (mémoire préliminaire). Rev Neurol 1959;101:3-15. PMID 14423403
82: Multisociety Task Force on Persistent Vegetative State. Medical aspects of the persistent vegetative state: statement of a multisociety task force. N Engl J Med 1994;330:1499-508. PMID 7818633
83: Munari M, Zucchetta P, Carollo C, et al. Confirmatory tests in the diagnosis of brain death: comparison between SPECT and contrast angiography. Crit Care Med 2005;33:2068-73. PMID 16148482
84: Nair-Collins M, Northrup J, Olcese J. Hypothalamic-pituitary function in brain death: a review. J Intensive Care Med 2016;31(1):41-50. PMID 24692211
85: Nakagawa TA, Ashwal S, Mathur M, et al. Guidelines for the determination of brain death in infants and children: an update of the 1987 Task Force recommendations. Crit Care Med 2011;39(9):2139-55. PMID 21849823
86: Nakagawa TA, Ashwal S, Mathur M, Mysore M; Committee For Determination Of Brain Death In Infants Children. Guidelines for the determination of brain death in infants and children: an update of the 1987 Task Force recommendations – Executive Summary. Ann Neurol 2012;71(4):573-85. PMID 22522447
87: Nanda A, Schmidley J. Diagnosing brain death – is it still clinical. (Abstract P03.114). Neurology 2007;68 (Suppl 1):A137.
88: Neavyn MJ, Stolbach A, Greer DM, et al. ACMT position statement: determining brain death in adults after drug overdose. J Med Toxicol 2017;13(3):271-3. PMID 28255927
89: Newberg A, Alavi A, van Rhijn S, Cotter A, Reilly P. Radiologic diagnosis of brain death. JAMA 2002;288:2121-2. PMID 12413369
90: Ormrod JA, Ryder T, Chadwick RJ, Bonner SM. Experiences of families when a relative is diagnosed brain stem dead: understanding of death, observation of brain stem death testing, and attitudes to organ donation. Anaesthesia 2005;60:1002-8. PMID 16179046
91: Ostermann ME, Young B, Sibbald WJ, Nicolle MW. Coma mimicking brain death following baclofen overdose. Intensive Care Med 2000;26:1144-6. PMID 11030173
92: Outwater KM, Rockoff MA. Diabetes insipidus accompanying brain death in children. Neurology 1984;34:1243-6. PMID 6540411
93: Pallis C, Harley DH. The ABC of brainstem death, 2nd ed. London: BMJ Publishers, 2009.
94: Petty GW, Mohr JP, Pedley TA, et al. The role of transcranial Doppler in confirming brain death: sensitivity, specificity, and suggestions for performance and interpretation. Neurology 1990;40:300-3. PMID 2405294
95: Pope TM. Brain death: legal duties to accommodate religious objections. Chest 2015;148(2):e69. PMID 26238851
96: Pope TM. Legal briefing: brain death and total brain failure. J Clin Ethics 2014;25(3):245-57. PMID 25192349
97: Posner JB. Coma and other states of consciousness: the differential diagnosis of brain death. Ann N Y Acad Sci 1978;315:215-27. PMID 284736
98: Power BM, Van Heerden PV. The physiological changes associated with brain death—current concepts and implications for treatment of the brain-dead organ donor. Anaesth Intensive Care 1995;23:26-36. PMID 7778744
99: Powers AD, Graeber MC, Smith RR. Transcranial Doppler ultrasonography in the determination of brain death. Neurosurgery 1989;24:884-9. PMID 2747862
100: Powner DJ, Darby JM. Current considerations in the issue of brain death. Neurosurgery 1999;45:1222-7. PMID 10549941
101: Powner DJ, Hernandez M, Rives TE. Variability among hospital policies for determining brain death in adults. Crit Care Med 2004;32:1284-8. PMID 15187507
102: President's Commission for the Study of Ethical Problems in Medicine and Biomedical and Behavioral Research. Defining death. Medical, ethical, and legal issues in the determination of death. Washington, DC: US Government Printing Office, 1981.
103: President’s Council on Bioethics. Controversies in the Determination of Death. A White Paper by the President’s Council on Bioethics. Washington, DC, 2008. bioethicsarchive.georgetown.edu Accessed January 2010.
104: Quesnel C, Fulgencio JP, Adrie C, et al. Limitations of computed tomographic angiography in the diagnosis of brain death. Intensive Care Med 2007;33(12):2129-35. PMID 17643226
105: Reid RH, Gulenchyn KY, Ballinger JR. Clinical use of technetium-99m HM-PAO for determination of brain death. J Nucl Med 1989;30:1621-6. PMID 2795202
106: Robey TE, Marcolini EG. Organ donation after acute brain death: addressing limitations of time and resources in the emergency department. Yale J Biol Med 2013;86(3):333-42. PMID 24058308
107: Rosner F. Definition of death in Jewish law. N Y State J Med 1983;83:973-8. PMID 6577328
108: Russell JA, Epstein LG, Greer DM, Kirschen M, Rubin MA, Lewis A. Brain death, the determination of brain death, and member guidance for brain death accommodation requests: AAN position statement. Neurology 2019;92(5):228-32. PMID 30602465
109: Schafer JA, Caronna JJ. Duration of apnea needed to confirm brain death. Neurology 1978;28:661-6. PMID 27735
110: Schroder R. Later changes in brain death: signs of partial recirculation. Acta Neuropathol (Berl) 1983;62:15-23. PMID 6659874
111: Scott JB, Gentile MA, Bennett SN, Couture M, MacIntyre NR. Apnea testing during brain death assessment: a review of clinical practice and published literature. Respir Care 2013;58(3):532-8. PMID 22709413
112: Shaner DM, Orr RD, Drought T, Miller RB, Siegel M. Really, most SINCERELY dead: Policy and procedure in the diagnosis of death by neurologic criteria. Neurology 2004;62:1683-6. PMID 15159462
113: Shappell CN, Frank JI, Husaroi K, Sanchez M, Goldenberg F, Ardelt A. Practice variability in brain death determination: a call to action. Neurology 2013;81(23):2009-2014. PMID 24198290
114: Shemie SD, Doig C, Dickens B, et al. Severe brain injury to neurological determination of death: Canadian forum recommendations. CMAJ 2006;174:S1-13. PMID 16534069
115: Shewmon DA. "Brainstem death," "brain death" and death: a critical re-evaluation of the purported equivalence. Issues Law Med 1998a;14:125-45. PMID 9807242
116: Shewmon DA. The brain and somatic integration: insights into the standard biological rationale for equating “brain death” with death. J Med Philos 2001;26(5):457-78. PMID 11588655
117: Shewmon DA, Shewmon ES. The semiotics of death and its medical implications. Adv Exper Med Biol 2004;550:89-114. PMID 15053428
118: Siminoff L, Burant C, Youngner SJ. Death and organ procurement: public beliefs and attitudes. Kennedy Inst Ethics J 2004;14:217-34. PMID 15495379
119: Spears WE, Lewis A, Bakkar A, et al. What does "brainstem death" mean? A review of international protocols. Can J Anaesth 2023;70(4):651-8. PMID 37131037
120: Sperling D. Maternal brain death. Am J Law Med 2004;30:453-500. PMID 15651556
121: Staworn D, Lewison L, Marks J, Turner G, Levin D. Brain death in pediatric intensive care unit patients: incidence, primary diagnosis, and the clinical occurrence of Turner's triad. Crit Care Med 1994;22:1301-5. PMID 8045150
122: Townsend MF 3rd, Richard JR, Witt MA. Artificially stimulated ejaculation in the brain-dead patient: a case report. Urology 1996;47:760-2. PMID 8650881
123: Turmel A, Roux A, Bojanowski MW. Spinal man after declaration of brain death. Neurosurgery 1991;28:298-301. PMID 1997902
124: Varelas PN, Kananeh M, Brady P, et al. The relationship between manifestation of diabetes insipidus and estimated glomerular filtration rate in brain death. Crit Care Med 2024;52(2):e58-66. PMID 37966309
125: Varelas PN, Rehman M, Abdelhak T, et al. Single brain death examination is equivalent to dual brain death examinations. Neurocrit Care 2011;15(3):547-53. PMID 21604079
126: Wahlster S, Wijdicks EF, Patel PV, et al. Brain death declaration: practices and perceptions worldwide. Neurology 2015;84(18):1870-9. PMID 25854866
127: Walker AE, Diamond EL, Moseley J. The neuropathological findings in irreversible coma: a critique of the "respirator." J Neuropathol Exp Neurol 1975;34:295-323. PMID 1055791
128: Wang HH, Varelas PN, Henderson GV, Wijdicks EF, Greer DM. Improving uniformity in brain death determination policies over time. Neurology 2017;88:562-8. PMID 28077490
129: Wang MY, Wallace P, Gruen JP. Brain death documentation: analysis and issues. Neurosurgery 2002;51:731-6. PMID 12188952
130: Webb AC, Samuels OB. Reversible brain death after cardiopulmonary arrest and induced hypothermia. Crit Care Med 2011;39(6):1538-42. PMID 21494112
131: Wijdicks EF. Determining brain death in adults. Neurology 1995;45:1003-11. PMID 7746373
132: Wijdicks EF. The diagnosis of brain death. N Engl J Med 2001a;344:1215-21. PMID 11309637
133: Wijdicks EF. Brain death worldwide: accepted fact but no global consensus in diagnostic criteria. Neurology 2002;58:20-5. PMID 11781400
134: Wijdicks EF, Manno EM, Holets SR. Ventilator self-cycling may falsely suggest patient effort during brain death determination. Neurology 2005;65:774. PMID 16157923
135: Wijdicks EF, Pfeifer EA. Neuropathology of brain death in the modern transplant era. Neurology 2008;70:1234-7. PMID 18256368
136: Wijdicks EF, Rabinstein AA, Manno EM, Atkinson JD. Pronouncing brain death: contemporary practice and safety of the apnea test. Neurology 2008;71:1240-4. PMID 18852438
137: Wijdicks EF, Varelas PN, Gronseth GS, Greer DM; American Academy of Neurology. Evidence-based guideline update: determining brain death in adults: report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology 2010;74(23):1911-8. PMID 20530327
138: Wilson K, Gordon L, Selby JB Jr. The diagnosis of brain death with Tc-99m HMPAO. Clin Nucl Med 1993;18:428-34. PMID 8508581
139: Wood KE, Becker BN, McCartney JG, D'Alessandro AM, Coursin DB. Care of the potential organ donor. N Engl J Med 2004;351(26):2730-9. PMID 15616207
140: Yee AH, Mandrekar J, Rabinstein AA, Wijdicks EF. Predictors of apnea test failure during brain death determination. Neurocrit Care 2010;12(3):352-5. PMID 20217276
141: Yoshikai T, Tahara T, Kuroiwa T, et al. Plain CT findings of brain death confirmed by hollow skull sign in brain perfusion SPECT. Radiat Med 1997;15:419-24. PMID 9495795
142: Youngner SJ, Landefeld CS, Coulton CJ, Juknialis BW, Leary M. "Brain death" and organ retrieval. A cross-sectional survey of knowledge and concepts among health professionals. JAMA 1989;261:2205-10. PMID 2926968
143: Zuckier LS. Radionuclide evaluation of brain death in the post-McMath era. J Nucl Med 2016;57(10):1560-8. PMID 27516449

Contributors

All contributors' financial relationships have been reviewed and mitigated to ensure that this and every other article is free from commercial bias.

Author

David M Greer MD

Dr. Greer of Boston University School of Medicine has no relevant financial relationships to disclose.
See Profile

Editor

Matthew Lorincz MD PhD

Dr. Lorincz of the University of Michigan has no relevant financial relationships to disclose.
See Profile

Former Authors

Melissa Mercado MD
James Bernat MD

Patient Profile

Age range of presentation: 0 months to 65+ years
Sex preponderance: male=female
Heredity: none
Population groups selectively affected: none selectively affected
Occupation groups selectively affected: none selectively affected

This is an article preview.
Start a Free Account
to access the full version.

Nearly 3,000 illustrations, including video clips of neurologic disorders.
Every article is reviewed by our esteemed Editorial Board for accuracy and currency.
Full spectrum of neurology in 1,200 comprehensive articles.
Listen to MedLink on the go with Audio versions of each article.

Questions or Comment?

MedLink®, LLC

3525 Del Mar Heights Rd, Ste 304
San Diego, CA 92130-2122

Toll Free (U.S. + Canada): 800-452-2400

US Number: +1-619-640-4660

Support: service@medlink.com

Editor: editor@medlink.com

ISSN: 2831-9125

Brain death/death by neurologic criteria

Associated Disorders

Apnea
Coma
Persistent vegetative state

Differential Diagnosis

depressant drug intoxication
profound neuromuscular blockade
Guillain-Barré syndrome
botulism causing profound paralysis
vegetative state

Also Relevant

Ethical issues in neurology
Metabolic encephalopathy and metabolic coma
Neuromyotonia and myokymia
Persistent vegetative state
Severe closed head injury

Clinical Categories

General Neurology

Patient Handouts

Web References

Guidelines

Clinical Trials

NIH: Brain Injury

Brain death/death by neurologic criteria …

Brain death/death by neurologic criteria

Introduction

Overview

Key points

Historical note and terminology

Clinical manifestations

Presentation and course

Prognosis and complications

Clinical vignette

Biological basis

Etiology and pathogenesis

Epidemiology

Prevention

Differential diagnosis

Diagnostic workup

Management

Special considerations

Pregnancy

Sperm retrieval

Anesthesia

References

Contributors

Author

Editor

Former Authors

Patient Profile

ICD & OMIM codes

This is an article preview.
Start a Free Account
to access the full version.

Questions or Comment?

Also in This Category

Neurogenetics and genetic and genomic testing

Acute traumatic spinal cord injury

Tumors of the skull base

Toxic and nutritional deficiency optic neuropathies

Nystagmus

Third nerve palsy

Outpatient rehabilitation of chronic neurologic conditions

Coma due to drug intoxication

Brain death/death by neurologic criteria

Introduction

Overview

Key points

Historical note and terminology

Clinical manifestations

Presentation and course

Prognosis and complications

Clinical vignette

Biological basis

Etiology and pathogenesis

Epidemiology

Prevention

Differential diagnosis

Diagnostic workup

Management

Special considerations

Pregnancy

Sperm retrieval

Anesthesia

References

Contributors

Author

Editor

Former Authors

Patient Profile

ICD & OMIM codes

This is an article preview. Start a Free Account to access the full version.

Questions or Comment?

Also in This Category

Neurogenetics and genetic and genomic testing

Acute traumatic spinal cord injury

Tumors of the skull base

Toxic and nutritional deficiency optic neuropathies

Nystagmus

Third nerve palsy

Outpatient rehabilitation of chronic neurologic conditions

Coma due to drug intoxication

This is an article preview.
Start a Free Account
to access the full version.