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Clostridium botulinum bacteria on egg yolk agar

Close view of a Petri dish, which contained a growth medium of egg yolk agar that had been inoculated with Clostridium botulinum bacteria and subsequently incubated for a 72-hour period. Note the presence of the "lipase reaction," which manifests as a shiny margin that surrounds each colony. (Source: Photography by Larry Stauffer, Oregon State Public Health Laboratory, 2002. Public Health Image Library ID# 1930, Centers for Disease Control and Prevention, Atlanta, Georgia. Public domain.)